Peptide Reconstitution Calculator
Calculate the resulting concentration after reconstituting any peptide vial. Enter your vial size and water volume to determine injection volume in mL and syringe units.
How to Reconstitute a Peptide Vial
- 1
Clean the vial tops
Wipe the tops of both the peptide vial and bacteriostatic water vial with alcohol swabs. Allow to air dry.
- 2
Draw bacteriostatic water
Using an insulin syringe, draw your desired volume of bacteriostatic water (typically 1-3 mL). Use the calculator above to determine the right amount.
- 3
Add water to the peptide vial
Insert the needle into the peptide vial and slowly release the water against the glass wall. Do not spray directly onto the powder.
- 4
Gently dissolve
Gently swirl the vial in a circular motion until the powder is fully dissolved. Never shake — this can damage the peptide.
- 5
Store properly
Refrigerate at 2-8°C (36-46°F). Most reconstituted peptides are stable for up to 28 days. Do not freeze.
Frequently asked questions
What is peptide reconstitution?
Reconstitution is the process of adding a solvent (usually bacteriostatic water) to a lyophilized (freeze-dried) peptide powder to create an injectable solution. The amount of solvent added determines the concentration of the final solution.
How much bacteriostatic water should I add?
The amount depends on your vial size and desired concentration. Common volumes are 1-3 mL. More water means a more dilute solution (easier to measure small doses). Less water means a more concentrated solution (fewer injections per vial). This calculator helps find the right volume.
How do I calculate dose volume after reconstitution?
Divide your desired dose by the concentration. For example, if you reconstituted a 5 mg vial with 2 mL of water, the concentration is 2.5 mg/mL. For a 250 mcg (0.25 mg) dose, you would draw 0.1 mL (10 units on an insulin syringe).